af2085 r d systems eomes mouse Search Results


brachyury antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology brachyury antibody
Cells were cultured in media supplemented with LIF and various concentrations of RA for 72 hours. (A) RNA was isolated and RT-PCR was performed on 5μg of total RNA to investigate expression of Fgf5, <t>Brachyury</t> and Gata6. (B–D) Real Time (Quantitative) RT-PCR was performed on 5μg of total RNA. mRNA levels of markers of cell differentiation (B) Nestin, (C) PDGFRα and (D) LamB1 were analyzed. RNA expression levels of Wt, RS Rex1−/− and Rex1 overexpressing cells were normalized to 36B4 RNA levels. Asterisks indicate significance where p < 0.05. E. Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Whole cell extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. Experiments were performed at least three times using independent RNA or protein preparations with similar results.
Brachyury Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/af2085+r+d+systems+eomes+mouse/brachyury+Antibody/pmc03098814-646-34-46
Average 94 stars, based on 1 article reviews
brachyury antibody - by Bioz Stars, 2026-10
94/100 stars
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kqp0719121 af2085 r d systems t brachyury rabbit  (R&D Systems)
96
R&D Systems kqp0719121 af2085 r d systems t brachyury rabbit
Cells were cultured in media supplemented with LIF and various concentrations of RA for 72 hours. (A) RNA was isolated and RT-PCR was performed on 5μg of total RNA to investigate expression of Fgf5, <t>Brachyury</t> and Gata6. (B–D) Real Time (Quantitative) RT-PCR was performed on 5μg of total RNA. mRNA levels of markers of cell differentiation (B) Nestin, (C) PDGFRα and (D) LamB1 were analyzed. RNA expression levels of Wt, RS Rex1−/− and Rex1 overexpressing cells were normalized to 36B4 RNA levels. Asterisks indicate significance where p < 0.05. E. Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Whole cell extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. Experiments were performed at least three times using independent RNA or protein preparations with similar results.
Kqp0719121 Af2085 R D Systems T Brachyury Rabbit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/af2085+r+d+systems+eomes+mouse/Human%2FMouse+Brachyury+Antibody/pm38261156-1022-51-53
Average 96 stars, based on 1 article reviews
kqp0719121 af2085 r d systems t brachyury rabbit - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
human/mouse brachyury antibody  (Bio-Techne corporation)
96
Bio-Techne corporation human/mouse brachyury antibody
Cells were cultured in media supplemented with LIF and various concentrations of RA for 72 hours. (A) RNA was isolated and RT-PCR was performed on 5μg of total RNA to investigate expression of Fgf5, <t>Brachyury</t> and Gata6. (B–D) Real Time (Quantitative) RT-PCR was performed on 5μg of total RNA. mRNA levels of markers of cell differentiation (B) Nestin, (C) PDGFRα and (D) LamB1 were analyzed. RNA expression levels of Wt, RS Rex1−/− and Rex1 overexpressing cells were normalized to 36B4 RNA levels. Asterisks indicate significance where p < 0.05. E. Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Whole cell extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. Experiments were performed at least three times using independent RNA or protein preparations with similar results.
Human/Mouse Brachyury Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/af2085+r+d+systems+eomes+mouse/Human%2FMouse+Brachyury+Antibody/bio-techne+corporation___af2085
Average 96 stars, based on 1 article reviews
human/mouse brachyury antibody - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


Cells were cultured in media supplemented with LIF and various concentrations of RA for 72 hours. (A) RNA was isolated and RT-PCR was performed on 5μg of total RNA to investigate expression of Fgf5, Brachyury and Gata6. (B–D) Real Time (Quantitative) RT-PCR was performed on 5μg of total RNA. mRNA levels of markers of cell differentiation (B) Nestin, (C) PDGFRα and (D) LamB1 were analyzed. RNA expression levels of Wt, RS Rex1−/− and Rex1 overexpressing cells were normalized to 36B4 RNA levels. Asterisks indicate significance where p < 0.05. E. Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Whole cell extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. Experiments were performed at least three times using independent RNA or protein preparations with similar results.

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: ANALYSIS OF REX1 (ZFP42) FUNCTION IN EMBRYONIC STEM CELL DIFFERENTIATION

doi: 10.1002/dvdy.22037

Figure Lengend Snippet: Cells were cultured in media supplemented with LIF and various concentrations of RA for 72 hours. (A) RNA was isolated and RT-PCR was performed on 5μg of total RNA to investigate expression of Fgf5, Brachyury and Gata6. (B–D) Real Time (Quantitative) RT-PCR was performed on 5μg of total RNA. mRNA levels of markers of cell differentiation (B) Nestin, (C) PDGFRα and (D) LamB1 were analyzed. RNA expression levels of Wt, RS Rex1−/− and Rex1 overexpressing cells were normalized to 36B4 RNA levels. Asterisks indicate significance where p < 0.05. E. Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Whole cell extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. Experiments were performed at least three times using independent RNA or protein preparations with similar results.

Article Snippet: The membranes were incubated with anti- Rex1 antibody (#ab28141, Abcam, Cambridge, MA), Oct4 antibody (#sc-8629, Santa Cruz Biotechnology, Santa Cruz, CA), Sox2 antibody (#01438, Stem Cell Technologies, Vancouver, BC), Pax6 antibody (#PRB-278P, Covance ), Brachyury antibody (#af2085, R&D Systemc Inc., Minneapolis, MN ), Gata4 antibody (#sc-1237, Santa Cruz Biotechnology, Santa Cruz, CA ), and Cyclin D2 antibody (#sc-593, Santa Cruz Biotechnology, Santa Cruz, CA ), followed by the appropriate secondary.

Techniques: Cell Culture, Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing, Quantitative RT-PCR, Cell Differentiation, RNA Expression, Western Blot, SDS Page

Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Embryoid body extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. ES cells cultured in the presence of RA were used as a positive control with 1/10 of the amount loaded to confirm linearity. Undifferentiated ES cells were used as negative control. This experiment was performed at least three times starting with cultured cells with similar results from all experiments.

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: ANALYSIS OF REX1 (ZFP42) FUNCTION IN EMBRYONIC STEM CELL DIFFERENTIATION

doi: 10.1002/dvdy.22037

Figure Lengend Snippet: Western blot analysis of Rex1−/− and Rex1 overexpressing ES cell lines. Embryoid body extracts (100 μg) were loaded onto SDS-PAGE gels and probed for Pax6, Brachyury and Gata4 expression. Actin was used as a loading control. ES cells cultured in the presence of RA were used as a positive control with 1/10 of the amount loaded to confirm linearity. Undifferentiated ES cells were used as negative control. This experiment was performed at least three times starting with cultured cells with similar results from all experiments.

Article Snippet: The membranes were incubated with anti- Rex1 antibody (#ab28141, Abcam, Cambridge, MA), Oct4 antibody (#sc-8629, Santa Cruz Biotechnology, Santa Cruz, CA), Sox2 antibody (#01438, Stem Cell Technologies, Vancouver, BC), Pax6 antibody (#PRB-278P, Covance ), Brachyury antibody (#af2085, R&D Systemc Inc., Minneapolis, MN ), Gata4 antibody (#sc-1237, Santa Cruz Biotechnology, Santa Cruz, CA ), and Cyclin D2 antibody (#sc-593, Santa Cruz Biotechnology, Santa Cruz, CA ), followed by the appropriate secondary.

Techniques: Western Blot, SDS Page, Expressing, Cell Culture, Positive Control, Negative Control